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KMID : 0376219650020010015
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Chonnam Medical Journal
1965 Volume.2 No. 1 p.15 ~ p.20
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Effect of Low Temperatures on Dysentery phage
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Abstract
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The author has studied on the stability of polyvalent dysentery phage particles, of which host bacteria is Shigella flekneri la, which were kept at various low temperatures and obtained the following results.
When Phage particles were kept at -60℃ and -40℃, it was destroyed by freezing and the phage was relatively stable if hard freezing does not occur. And also, the phage particles did not receive any effect at -8℃.
The more the manipulation of freezing and thawing was repeated, the more phage particle´s were destroyed and the phage repeatedly froze and thawed at -60℃ was more destroyed than that repeatedly froze and thawed at -40℃.
At -8℃, the phage particles did not receive any damage even though the manipulation of freezing and thawing was performed three times.
When phage particles were destroyed by freezing and thawing, the more the freezing is rapid, the more the phage particles were destroyed and the rapidity of thawing was seemed to give no effect on phage particles.
The mechanism by which phage particles were destroyed during freezing and thawing was discussed.
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